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ORIGINAL ARTICLE
Year : 2017  |  Volume : 7  |  Issue : 4  |  Page : 181-187

An In vitro assessment of antibacterial activity of three self-etching primers against oral microflora


Department of Orthodontics and Dentofacial Orthopedics, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India

Correspondence Address:
Sneha Dipak Shinde
PG Resident, Department of Orthodontics and Dentofacial Orthopedics, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Bauxite Road, Belagavi, Karnataka
India
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/apos.apos_11_17

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Aims: This study aims to evaluate and compare the antibacterial activity of three self-etching primers (SEP), namely, Transbond plus, Reliance, and Gluma against commonly encountered oral microflora (Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus). Subjects and Methods: The antibacterial activity of the three SEPs was examined against microorganisms using agar diffusion test (ADT) and minimum inhibitory concentration (MIC). In ADT, Whatman's filter paper disc of 5 mm was loaded with primer and polymerized. This was placed on previously inoculated brain heart infusion and blood agar plates and was incubated for 48–72 h at 37°C according to the microorganism. For assessing MIC serial dilution method was used. Statistical Analysis Used: Data were analyzed with Kruskal–Wallis (P < 0.001) and Mann–Whitney tests. Results: Only Transbond plus SEP and Reliance SEP produced a clear growth inhibition halo against S. mutans, L. acidophilus and A. viscosus. Gluma SEP did not show any growth inhibition halo against S. mutans, L. acidophilus, and A. viscosus. Conclusions: TSEP and Reliance SEP did show antibacterial activity in an in vitro environment. Therefore, this study concludes that the use of these SEPs may contribute to a reduction in bacterial colonization.


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